概述

  • 产品名称Anti-VDAC2抗体
    参阅全部 VDAC2 一抗
  • 描述
    山羊多克隆抗体to VDAC2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-GHKVGLALELEA

    , corresponding to C terminal amino acids 283-294 of Human VDAC2

  • 阳性对照
    • Human Brain and Testes lysates.

性能

应用

Our Abpromise guarantee covers the use of ab37985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
  • 组织特异性Expressed in all tissues examined.
  • 序列相似性Belongs to the eukaryotic mitochondrial porin family.
  • 结构域Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
  • 细胞定位Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B-36 vdac antibody
    • FLJ23841 antibody
    • hVDAC2 antibody
    • OTTHUMP00000019878 antibody
    • OTTHUMP00000019879 antibody
    • OTTHUMP00000019880 antibody
    • Outer mitochondrial membrane protein porin 2 antibody
    • POR antibody
    • Porin-2 antibody
    • VDAC 2 antibody
    • VDAC-2 antibody
    • VDAC2 antibody
    • VDAC2_HUMAN antibody
    • Vdac6 antibody
    • Voltage dependent anion channel 2 antibody
    • Voltage dependent anion selective channel protein 2 antibody
    • Voltage-dependent anion-selective channel protein 2 antibody
    see all

Anti-VDAC2 antibody 图像

  • Anti-VDAC2 antibody (ab37985) at 1 µg/ml + Human Brain (Cerebellum) Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa
  • ICC/IF image of ab37985 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37985, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-VDAC2 antibody (ab37985) at 1 µg/ml + Human Testis lysate (35µg protein in RIPA buffer).

    Predicted band size : 32 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-VDAC2 antibody (ab37985)参考文献

This product has been referenced in:
  • Naghdi S  et al. Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis. Proc Natl Acad Sci U S A 112:E5590-9 (2015). WB . Read more (PubMed: 26417093) »
  • Falvella FS  et al. Multiple isoforms and differential allelic expression of CHRNA5 in lung tissue and lung adenocarcinoma. Carcinogenesis 34:1281-5 (2013). WB ; Human . Read more (PubMed: 23430818) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us. Unfortunately, both VDAC1 and VDAC3 are very homologous with VDAC2. While we have not tested these antibodies specifically for cross-reactivity with VDAC1 and VDAC3, the antibodies ab37985 and ab118872 show ˜67% homology wi...

Read More
Application Western blot
Sample Rat Tissue lysate - other (Mitochondrial fraction from skeletal muscle)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 15 µg
Specification Mitochondrial fraction from skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Application Western blot
Sample Mouse Tissue lysate - other (Mitochondrial fraction from skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 15 µg
Specification Mitochondrial fraction from skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Skeletal muscle)
Loading amount 30 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 Sep 28 2012

Thank you so much.

Please let me know if there is anything else I can help you with.

Thank you for contacting us.

We have the following antibodies for VDAC2 and VDAC3 that have been tested in WB and with human samples:

VDAC2: ab47104, ab37985, ab118872, ab100956, ab77160, ab117587, ab126120

VDAC3: ab3745...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"