The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Dot: 1/2000, determined using rat liver mitochondria extract.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
组织特异性Heart, liver and skeletal muscle.
序列相似性Belongs to the eukaryotic mitochondrial porin family.
结构域Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.