Anti-VCP抗体[Hs-14] (FITC) (ab77238)


  • 产品名称
    Anti-VCP抗体[Hs-14] (FITC)
    参阅全部 VCP 一抗
  • 描述
    小鼠单克隆抗体[Hs-14] to VCP (FITC)
  • 宿主
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Tissue, cells or virus corresponding to Human VCP. Freshly ejaculated human sperm washed in PBS & extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and then used for immunization.

  • 阳性对照
    • Sperm cells.
  • 常规说明

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.



Our Abpromise guarantee covers the use of ab77238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明
    Flow Cyt: Use 20µl for 100µl cell suspension.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
    • 疾病相关
      Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
    • 序列相似性
      Belongs to the AAA ATPase family.
    • 翻译后修饰
      Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位
      Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 15S Mg(2+) ATPase p97 subunit antibody
      • 15S Mg(2+)-ATPase p97 subunit antibody
      • ALS14 antibody
      • ATPase p97 antibody
      • CDC48 antibody
      • IBMPFD antibody
      • MGC131997 antibody
      • MGC148092 antibody
      • MGC8560 antibody
      • p97 antibody
      • TER ATPase antibody
      • TERA antibody
      • TERA_HUMAN antibody
      • Transitional endoplasmic reticulum ATPase antibody
      • Valosin containing protein antibody
      • Valosin-containing protein antibody
      • VCP antibody
      • Yeast Cdc48p homolog antibody
      see all


    This product has been referenced in:
    • Peknicova J  et al. Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol 53:42-9 (2005). Read more (PubMed: 15667524) »
    • Chládek D  et al. [Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization] Ceska Gynekol 65:28-32 (2000). Read more (PubMed: 10750294) »

    See all 2 Publications for this product


    The concentration is 190 ug/ml.