概述

  • 产品名称Anti-VCP抗体[EPR3308]
    参阅全部 VCP 一抗
  • 描述
    兔单克隆抗体[EPR3308] to VCP
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • MCF-7, A549 and RAW264.7 cell lysates; Human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
    • 疾病相关Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
    • 序列相似性Belongs to the AAA ATPase family.
    • 翻译后修饰Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • 细胞定位Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 15S Mg(2+) ATPase p97 subunit antibody
      • 15S Mg(2+)-ATPase p97 subunit antibody
      • ALS14 antibody
      • ATPase p97 antibody
      • CDC48 antibody
      • IBMPFD antibody
      • MGC131997 antibody
      • MGC148092 antibody
      • MGC8560 antibody
      • p97 antibody
      • TER ATPase antibody
      • TERA antibody
      • TERA_HUMAN antibody
      • Transitional endoplasmic reticulum ATPase antibody
      • Valosin containing protein antibody
      • Valosin-containing protein antibody
      • VCP antibody
      • Yeast Cdc48p homolog antibody
      see all

    Anti-VCP antibody [EPR3308] 图像

    • All lanes : Anti-VCP antibody [EPR3308] (ab109198) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 89 kDa
    • ab109198, at 1/50 dilution, staining VCP in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Anti-VCP antibody [EPR3308] (ab109198)参考文献

    This product has been referenced in:
    • Wang T  et al. Interaction of amyotrophic lateral sclerosis/frontotemporal lobar degeneration-associated fused-in-sarcoma with proteins involved in metabolic and protein degradation pathways. Neurobiol Aging N/A:N/A (2014). Read more (PubMed: 25192599) »

    See 1 Publication for this product

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