概述

  • 产品名称Anti-VCP抗体
    参阅全部 VCP 一抗
  • 描述
    兔多克隆抗体to VCP
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 290 and 507 of human VCP (P55072)

  • 阳性对照
    • MCF7, U87-MG and NT2D1 whole cells lysates; IMR32 cell lysate; MCF7 cells This antibody gave a positive result in IHC in the following FFPE tissue: Human Liver.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab111740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
  • 疾病相关Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
  • 序列相似性Belongs to the AAA ATPase family.
  • 翻译后修饰Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 15S Mg(2+) ATPase p97 subunit antibody
    • 15S Mg(2+)-ATPase p97 subunit antibody
    • ALS14 antibody
    • ATPase p97 antibody
    • CDC48 antibody
    • IBMPFD antibody
    • MGC131997 antibody
    • MGC148092 antibody
    • MGC8560 antibody
    • p97 antibody
    • TER ATPase antibody
    • TERA antibody
    • TERA_HUMAN antibody
    • Transitional endoplasmic reticulum ATPase antibody
    • Valosin containing protein antibody
    • Valosin-containing protein antibody
    • VCP antibody
    • Yeast Cdc48p homolog antibody
    see all

Anti-VCP antibody 图像

  • All lanes : Anti-VCP antibody (ab111740) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : U87-MG whole cell lysate
    Lane 3 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 89 kDa
    7.5% SDS PAGE
  • IHC image of VCP staining in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab111740, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence analysis of VCP in paraformaldehyde fixed MCF7, using ab111740 at a 1/500 dilution. Lower image shows cells costained with Hoechst 33342.

Anti-VCP antibody (ab111740)参考文献

ab111740 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Chlamydomonas reinhardtii Cell lysate - whole cell (stationary phase)
Specification stationary phase
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Yuqing Hou

Verified customer

提交于 Jun 05 2014

Abreviews
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (gel 10 %)
Sample Plants Cell lysate - whole cell (tobacco cells in suspension)
Specification tobacco cells in suspension
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Ms. Claire Rosnoblet

Verified customer

提交于 Oct 15 2013

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