The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 20µl for 106 cells.
or 100 µl of whole blood
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.