PE Anti-VCAM1抗体[M/K-2] (ab25452)
Key features and details
- PE Rat monoclonal [M/K-2] to VCAM1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
概述
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产品名称
PE Anti-VCAM1抗体[M/K-2]
参阅全部 VCAM1 一抗 -
描述
PE大鼠单克隆抗体[M/K-2] to VCAM1 -
宿主
Rat -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab25452 recognises CD106/VCAM1 (100 kDa) -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to VCAM1. BALB/3T3 and +/+2.4 cells
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阳性对照
- Flow Cyt: Mouse bone marrow cells.
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常规说明
The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading...
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纯度
Affinity purified -
Primary antibody说明
The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions. -
克隆
单克隆 -
克隆编号
M/K-2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 0.2µg for 106 cells.
ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 0.2µg for 106 cells. ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
组织特异性
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
序列相似性
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
翻译后修饰
Sialoglycoprotein. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 22329 Mouse
- SwissProt: P29533 Mouse
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
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别名
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab25452 被引用在 1 文献中.
- Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . PubMed: 23806234