This antibody gave a positive signal in the following tissue lysates: Mouse Spleen; Rat Spleen; Mouse Thymus; Rat Thymus; Mouse Lung.
This antibody gave a positive result when used in the following methanol fixed cell lines: NIH-3T3
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).
Use a concentration of 5 µg/ml.
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
ICC/IF image of ab115135 stained NIH-3T3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115135 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-VCAM1 antibody (ab115135)
All lanes : Anti-VCAM1 antibody (ab115135) at 1 µg/ml
Lane 1 : Spleen (Mouse) Tissue Lysate Lane 2 : Spleen (Rat) Tissue Lysate Lane 3 : Thymus (Mouse) Tissue Lysate Lane 4 : Thymus (Rat) Tissue Lysate Lane 5 : Mouse lung normal tissue lysate - total protein (ab29297)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique