概述

  • 产品名称
  • 描述
    兔多克隆抗体to VCAM1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit
  • 免疫原

    Recombinant fragment, corresponding to amino acids 195-474 of Human VCAM1 (BC068490).

  • 阳性对照
    • HeLa cell lysate, Human fetal spleen, kidney and heart lysates; Human fetal skin tissue.

性能

应用

Our Abpromise guarantee covers the use of ab106777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 81 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能
    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 组织特异性
    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 序列相似性
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域
    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻译后修饰
    Sialoglycoprotein.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

图片

  • ab106777 at 1/100 dilution, staining VCAM1 in Human fetal skin by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

  • All lanes : Anti-VCAM1 antibody (ab106777) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal spleen lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal heart lysate


    Predicted band size : 81 kDa

文献

This product has been referenced in:
  • Carnuta MG  et al. Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients. Sci Rep 7:7295 (2017). Read more (PubMed: 28779156) »
  • Xiao Y  et al. Effect of a-linolenic acid-modified low molecular weight chondroitin sulfate on atherosclerosis in apoE-deficient mice. Biochim Biophys Acta 1860:2589-97 (2016). Read more (PubMed: 27491336) »

See all 5 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (umbilical cord)
Antigen retrieval step
None
Permeabilization
No
Specification
umbilical cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 29 2016

Abreviews
Application
ELISA
Sample
Human Serum (cardiovascular biomarker)
Specification
cardiovascular biomarker
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

提交于 Mar 13 2013

Reply to CCE ******* and CCE ******** as I assume both complaints are from the same customer.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear both antibodies ab41532 and ab106777 are not provi...

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Thank you for your reply regarding ab106777.


I am happy to hear that this antibody worked successfully for you in rat. In order to validate the discount code, please submit an Abreview through our website:


http://www.abcam.c...

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DISCOUNT CODE: ***********
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab106777 in rat tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To rede...

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Thank you very much for your interest in ab106777.

To our knowledge, ab106777 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab106777 now, test it in rat and submit feedback to us in the form o...

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I can confirm that we didn't test the antibody (ab106777: Anti-VCAM1) in Rabbit tissue, as soon as we have further information we will update our on-line datasheet accordingly.

Thank you for your interest.



The reactivity of this antibody (ab2213) with rabbit samples on Western blot application is probable but it has not been tested yet.



Hope this will be useful for you!

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Primary Endothelial cells)
Loading amount
5 µg
Specification
Primary Endothelial cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. S Maalouf

Verified customer

提交于 Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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