概述

  • 产品名称
    Anti-VCAM1抗体[6G9]
    参阅全部 VCAM1 一抗
  • 描述
    小鼠单克隆抗体[6G9] to VCAM1
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Rabbit, Human
  • 免疫原

    Purified recombinant fragment of Human VCAM1 expressed in E. Coli.

  • 阳性对照
    • HUVEC and EC cell lysate. Human lung cancer and colon cancer tissue.

性能

应用

Our Abpromise guarantee covers the use of ab98954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 81 kDa.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.

靶标

  • 功能
    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 组织特异性
    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 序列相似性
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域
    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻译后修饰
    Sialoglycoprotein.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

图片

  • All lanes : Anti-VCAM1 antibody [6G9] (ab98954) at 1/500 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : EC cell lysate

    Predicted band size: 81 kDa

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue (1) and colon cancer tissue (2) using ab98954 at 1/200 dilution with DAB staining.

文献

This product has been referenced in:
  • Kotha S  et al. Microvasculature-directed thrombopoiesis in a 3D in vitro marrow microenvironment. PLoS One 13:e0195082 (2018). ICC/IF . Read more (PubMed: 29617409) »
  • Valero-Muñoz M  et al. Relevance of vascular peroxisome proliferator-activated receptor ? coactivator-1a to molecular alterations in atherosclerosis. Exp Physiol 98:999-1008 (2013). Read more (PubMed: 23335007) »

See all 4 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (vascular tissue)
Loading amount
40 µg
Specification
vascular tissue
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: RT°C
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提交于 Feb 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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