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Full length protein corresponding to VCAM1.
Our Abpromise guarantee covers the use of ab7224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab7224 staining VCAM1 in Human pluripotent stem cell derived cardiomyocytes by Flow Cytometry. Cells were cultured in a monolayer with PBS + 2% FBS. The sample was incubated with the primary antibody (1/100 in PBS and 2% FBS) for 30 minutes at 4°C. A Biotin-conjugated mouse IgG (1/1000) was used as the secondary antibody.
Gating Strategy: Negative control population of pluripotent stem cells.