Anti-VCAM1抗体[1G11B1] (ab7219)

概述

  • 产品名称Anti-VCAM1抗体[1G11B1]
    参阅全部 VCAM1 一抗
  • 描述
    小鼠单克隆抗体[1G11B1] to VCAM1
  • 特异性The antibody is extremely useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. The antibody can be used for flow cytometry and immunoprecipitation.
  • 经测试应用适用于: IP, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human).

  • 阳性对照
    • Lymph nodes can be used as positive control. As negative control it is advised to use as first antibody a control murine IgG1 antibody.

应用

Our Abpromise guarantee covers the use of ab7219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
The antibody is extremely useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. The antibody can be used for flow cytometry and immunoprecipitation. In vitro cultured cells can be fixed with 1% paraformaldehyde. Tissue sections should be fixed for 10 min in pure acetone followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised followed by a biotin-conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation, a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 8 hours with TNF alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ug/ml) in tiss
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 组织特异性Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 序列相似性Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻译后修饰Sialoglycoprotein.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

Anti-VCAM1 antibody [1G11B1] (ab7219)参考文献

ab7219 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human pluripotent SC derived epicardial like cells)
Permeabilization Yes - Saponin
Specification Human pluripotent SC derived epicardial like cells
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative Paraformaldehyde
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提交于 Oct 31 2016

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This antibody has not been tested on chicken, therefore we can't guarantee results.

Unfortunately, this data has not yet been determined. Our apologies.

This antibody recognises the human form of V-CAM1. To our knowledge, it has not been tested on mouse tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"