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RabMAb

Anti-VAV3抗体[EP1130Y] (ab52938)

概述

  • 产品名称
    Anti-VAV3抗体[EP1130Y]
    参阅全部 VAV3 一抗
  • 描述
    兔单克隆抗体[EP1130Y] to VAV3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide within Human VAV3 (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • Jurkat cell lysate; permeabalized Jurkat cells.
  • 常规说明

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IP 1/50.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 23566222

Methanol fixed cells

  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
    • 序列相似性
      Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ40431 antibody
      • Guanine nucleotide exchange factor VAV3 antibody
      • Protein vav 3 antibody
      • Protein vav3 antibody
      • RGD1565941 antibody
      • VAV 3 antibody
      • Vav 3 guanine nucleotide exchange factor antibody
      • VAV 3 oncogene antibody
      • VAV 3 protein antibody
      • VAV-3 antibody
      • Vav3 antibody
      • VAV3 oncogene antibody
      • VAV3 protein antibody
      • VAV3_HUMAN antibody
      see all

    图片

    • Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 98 kDa
      Observed band size: ~98 kDa (why is the actual band size different from the predicted?)

    • Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).

    文献

    This product has been referenced in:

    See all 4 Publications for this product

    客户评价及客户问答

    Thanks for the reply. I am a little stumped as to why you are having these issues. I can only think of 2 things that may help (and I am sure you have already tried them): 1 - One reason for multiply bands is that there is not enough protease inhibitor ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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