Anti-VAV2 (phospho Y142)抗体(ab73707)

概述

  • 产品名称Anti-VAV2 (phospho Y142)抗体
    参阅全部 VAV2 一抗
  • 描述
    兔多克隆抗体to VAV2 (phospho Y142)
  • 特异性This antibody detects endogenous levels of VAV2 only when phosphorylated at tyrosine 142.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthesized phosphopeptide derived from human VAV2 around the phosphorylation site of tyrosine 142 (D-V-YP-R-S).

  • 阳性对照
    • Human brain tissue sections, NIH/3T3 cells and extracts from K562 cells and Hela cells, treated with TNF.

性能

应用

Our Abpromise guarantee covers the use of ab73707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
  • 组织特异性Widely expressed.
  • 序列相似性Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Guanine nucleotide exchange factor VAV2 antibody
    • Oncogene VAV2 antibody
    • Protein vav 2 antibody
    • VAV 2 antibody
    • Vav 2 oncogene antibody
    • VAV-2 antibody
    • VAV2 antibody
    • VAV2 oncogene antibody
    • VAV2_HUMAN antibody
    see all

Anti-VAV2 (phospho Y142) antibody 图像

  • All lanes : Anti-VAV2 (phospho Y142) antibody (ab73707) at 1/500 dilution

    Lane 1 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins)
    Lane 2 : Extracts from Hela cells treated with TNF (200ng/ml, 30mins)
    Lane 3 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins) plus 5µg immunizing phosphopeptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab73707 at a 1:50 dilution.

    Left image un-treated.

    Right image treated with immunizing phosphopeptide.

  • Immunofluorescence analysis of NIH/3T3 cells using ab73707 at a 1:500 dilution.

    Left image un-treated.

    Right image treated with immunizing phosphopeptide.

Anti-VAV2 (phospho Y142) antibody (ab73707)参考文献

ab73707 has not yet been referenced specifically in any publications.

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