The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Immunocytochemical labeling of VAV2 in paraformaldehyde-fixed and NP40 permeabilized rabbit spleen fibroblasts. The cells were labeled with ab86699 at a 1/50 dilution and detected using an appropriate secondary antibody conjugated to Cy3.