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RabMAb

Anti-VAV1 (phospho Y174)抗体[EP510Y] (ab76225)

概述

  • 产品名称
    Anti-VAV1 (phospho Y174)抗体[EP510Y]
    参阅全部 VAV1 一抗
  • 描述
    兔单克隆抗体[EP510Y] to VAV1 (phospho Y174)
  • 特异性
    This antibody detects VAV1 phosphorylated at Tyrosine 174.
  • 经测试应用
    适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VAV1 (phospho Y174). The exact sequence is proprietary.

  • 阳性对照
    • WB: Jurkat cell lysate treated with pervanadate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab76225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250000 - 1/500000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
  • 应用说明
    Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能
      Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
    • 组织特异性
      Widely expressed in hematopoietic cells but not in other cell types.
    • 序列相似性
      Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • 结构域
      The DH domain is involved in interaction with CCPG1.
    • 翻译后修饰
      Phosphorylated on tyrosine residues.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Oncogene vav antibody
      • p95Vav antibody
      • Proto-oncogene vav antibody
      • Protooncogene vav antibody
      • VAV 1 antibody
      • VAV 1 oncogene antibody
      • VAV antibody
      • Vav proto oncogene antibody
      • VAV_HUMAN antibody
      • VAV1 antibody
      see all

    Anti-VAV1 (phospho Y174) antibody [EP510Y] 图像

    • Immunocytochemistry/Immunofluorescence analysis of Untreated Jurkat cells and Pervanadate (1mM,30min) treated Jurkat cells labelling VAV1 (phospho Y174) with purified ab76225 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1:1000 (1ug/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Negative Control 1: Rabbit primary antibody and anti-mouse secondary antibody(ab150120)

      Negative Control 2: Mouse primary antibody(ab7291) and anti-rabbit secondary antibody(ab150077)

    • All lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/500000 dilution

      Lane 1 : Untreated Jurkat cell lysate
      Lane 2 : Jurkat cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 101 kDa
      Observed band size : 101 kDa
    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).

    • Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225)参考文献

    This product has been referenced in:
    • Parikh K  et al. Suppression of p21Rac signaling and increased innate immunity mediate remission in Crohn's disease. Sci Transl Med 6:233ra53 (2014). Read more (PubMed: 24760188) »
    • Wakahashi S  et al. VAV1 represses E-cadherin expression through the transactivation of Snail and Slug: a potential mechanism for aberrant epithelial to mesenchymal transition in human epithelial ovarian cancer. Transl Res 162:181-90 (2013). WB ; Human . Read more (PubMed: 23856093) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    12 µg
    Gel Running Conditions
    Reduced Denaturing (gel 8%)
    Sample
    Human Cell lysate - whole cell (SKOV3 cells were transfected with VAV1)
    Specification
    SKOV3 cells were transfected with VAV1
    Blocking step
    Blocking one (nacalai tesque) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
    Username

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    Verified customer

    提交于 Jul 18 2013

    The difference between these two products is that ab76225 is a rabbit monoclonal antibody raised while ab47282 is a rabbit polyclonal both of these antibodies were raised against the corresponding residues around the phophorylated site at the 174 amino...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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