RabMAb

Anti-Vav proteins抗体[EP482Y] (ab40875)

概述

  • 产品名称Anti-Vav proteins抗体[EP482Y]
    参阅全部 Vav proteins 一抗
  • 描述
    兔单克隆抗体[EP482Y] to Vav proteins
  • 经测试应用适用于: WB, Flow Cyt, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human Vav.

  • 阳性对照
    • Jurkat cells lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab40875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50.
IP 1/50.

靶标

  • 相关性The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.
  • 细胞定位Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
  • 数据库链接
  • 别名
    • 2810040F13Rik antibody
    • A530094I06Rik antibody
    • AA986410 antibody
    • AI847175 antibody
    • FLJ40431 antibody
    • GDP/GTP exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV3 antibody
    • MGC27838 antibody
    • Oncogene VAV antibody
    • Oncogene VAV1 antibody
    • Oncogene VAV2 antibody
    • Oncogene VAV3 antibody
    • p95 antibody
    • p95vav antibody
    • Protein vav 1 antibody
    • Protein vav 2 antibody
    • Protein vav 3 antibody
    • Proto oncogene vav antibody
    • RGD1565941 antibody
    • VAV antibody
    • vav 1 guanine nucleotide exchange factor antibody
    • Vav 1 oncogene antibody
    • vav 2 guanine nucleotide exchange factor antibody
    • Vav 2 oncogene antibody
    • vav 3 guanine nucleotide exchange factor antibody
    • Vav 3 oncogene antibody
    • vav-T antibody
    • VAV1 oncogene antibody
    • VAV2 oncogene antibody
    • VAV3 oncogene antibody
    see all

Anti-Vav proteins antibody [EP482Y] 图像

  • Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate (10 ug).

    Predicted band size : 98 kDa
    Observed band size : 98 kDa
  • Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Vav proteins antibody [EP482Y] (ab40875)参考文献

This product has been referenced in:
  • Gastaldello S  et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . Read more (PubMed: 24130483) »
  • Pauker MH  et al. Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal 5:rs3 (2012). IP . Read more (PubMed: 22534133) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Loading amount 25 µg
Specification platelet
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 3% · Temperature: 23°C
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提交于 Aug 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"