Anti-VASP (phospho S157)抗体[5C6] (ab58555)

概述

  • 产品名称Anti-VASP (phospho S157)抗体[5C6]
    参阅全部 VASP 一抗
  • 描述
    小鼠单克隆抗体[5C6] to VASP (phospho S157)
  • 特异性This antibody recognizes the the ~50 kDa VASP protein phosphorylated at Ser157. It does not cross-react with non-phosphorylated VASP or with un-related phosphorylation sites.
  • 经测试应用适用于: Flow Cyt, WB, IP, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic phosphopeptide corresponding to amino acids surrounding the Ser157 phosphorylation site of human VASP

  • 阳性对照
    • Vanadate-treated A431 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 2 x PBS, Polyethylene glycol, Sucrose
  • Concentration information loading...
  • 纯度Size exclusion
  • 纯化说明Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography.
  • 克隆单克隆
  • 克隆编号5C6
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
IP Use 1-10µg for 106 cells.
ELISA Use a concentration of 0.05 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde.

靶标

  • 功能Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
  • 组织特异性Highly expressed in platelets.
  • 序列相似性Belongs to the Ena/VASP family.
    Contains 1 WH1 domain.
  • 结构域The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    The WH1 domain mediates interaction with XIRP1.
  • 翻译后修饰Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Vasodilator stimulated phosphoprotein antibody
    • Vasodilator-stimulated phosphoprotein antibody
    • VASP antibody
    • VASP_HUMAN antibody

Anti-VASP (phospho S157) antibody [5C6] 图像

  • Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-VASP (phospho S157) antibody [5C6] (ab58555)参考文献

This product has been referenced in:
  • Neukamm SS  et al. Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation. J Biol Chem 288:16403-15 (2013). Human . Read more (PubMed: 23615913) »
  • Mise N  et al. Zyxin is a transforming growth factor-ß (TGF-ß)/Smad3 target gene that regulates lung cancer cell motility via integrin a5ß1. J Biol Chem 287:31393-405 (2012). WB . Read more (PubMed: 22778267) »

See all 2 Publications for this product

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I have contacted the lab on your behalf, the antibody would be fine as well in a different buffer containing another stabilizing agent e.g. BSA. However this product is provided in PEG and suc...

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