Anti-VASP (phospho S156)抗体[EPR1065(2)] (ab109541)


  • 产品名称Anti-VASP (phospho S156)抗体[EPR1065(2)]
    参阅全部 VASP 一抗
  • 描述
    兔单克隆抗体[EPR1065(2)] to VASP (phospho S156)
  • 特异性ab109541 only detects VASP phosphorylated at Serine 156.
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Serine 156 of Human VASP.

  • 阳性对照
    • Human platelet cell lysate; Human kidney tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab109541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
    • 组织特异性Highly expressed in platelets.
    • 序列相似性Belongs to the Ena/VASP family.
      Contains 1 WH1 domain.
    • 结构域The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
      The WH1 domain mediates interaction with XIRP1.
    • 翻译后修饰Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
    • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Vasodilator stimulated phosphoprotein antibody
      • Vasodilator-stimulated phosphoprotein antibody
      • VASP antibody
      • VASP_HUMAN antibody

    Anti-VASP (phospho S156) antibody [EPR1065(2)] 图像

    • All lanes : Anti-VASP (phospho S156) antibody [EPR1065(2)] (ab109541) at 1/1000 dilution

      Lane 1 : Human platelet cell lysate, untreated
      Lane 2 : Human platelet cell lysate treated with Alkaline Phosphatase (AP)

      Lysates/proteins at 10 µg per lane.

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
    • ab109541 at 1/100 dilution staining VASP (phospho S156) in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Anti-VASP (phospho S156) antibody [EPR1065(2)] (ab109541)参考文献

    ab109541 has not yet been referenced specifically in any publications.

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