概述

  • 产品名称Anti-VASP抗体
    参阅全部 VASP 一抗
  • 描述
    小鼠多克隆抗体to VASP
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human VASP protein (AAH38224.1).

  • 阳性对照
    • Human spleen tissue lysate. Cell lysate from transfected 293T cells. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab89829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
  • 组织特异性Highly expressed in platelets.
  • 序列相似性Belongs to the Ena/VASP family.
    Contains 1 WH1 domain.
  • 结构域The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    The WH1 domain mediates interaction with XIRP1.
  • 翻译后修饰Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Vasodilator stimulated phosphoprotein antibody
    • Vasodilator-stimulated phosphoprotein antibody
    • VASP antibody
    • VASP_HUMAN antibody

Anti-VASP antibody 图像

  • Anti-VASP antibody (ab89829) at 1 µg/ml + human spleen tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • All lanes : Anti-VASP antibody (ab89829) at 1 µg/ml

    Lane 1 : cell lysate from VASP transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab89829 at 10 µg/ml staining VASP in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

Anti-VASP antibody (ab89829)参考文献

ab89829 has not yet been referenced specifically in any publications.

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