Anti-Vasopressin V1b receptor抗体(ab104365)

概述

  • 产品名称Anti-Vasopressin V1b receptor抗体
    参阅全部 Vasopressin V1b receptor 一抗
  • 描述
    兔多克隆抗体to Vasopressin V1b receptor
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Vasopressin V1b receptor.

  • 阳性对照
    • This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue lysates: E10 Mouse Embryo Brain; Mouse Forebrain; Human Brain Membrane

性能

应用

Our Abpromise guarantee covers the use of ab104365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antidiuretic hormone receptor 1b antibody
    • Arginine vasopressin receptor 1B antibody
    • AVP R3 antibody
    • AVPR 1B antibody
    • AVPR 3 antibody
    • AVPR V1b antibody
    • AVPR V3 antibody
    • Avpr1b antibody
    • AVPR3 antibody
    • V1bR antibody
    • V1BR_HUMAN antibody
    • Vasopressin V1b receptor antibody
    • Vasopressin V3 receptor antibody
    • VPR 3 antibody
    • VPR3 antibody
    see all

Anti-Vasopressin V1b receptor antibody 图像

  • All lanes : Anti-Vasopressin V1b receptor antibody (ab104365) at 1 µg/ml

    Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 2 : Forebrain (Mouse) Tissue Lysate
    Lane 3 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : E10 Mouse Embryo Brain Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : Forebrain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 7 : Human brain normal tissue lysate - membrane extract (ab29456) with Immunising peptide at 1 µg/ml
    Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 102 kDa,22 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab104365 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) SKNSH cells at 1µg/ml.

Anti-Vasopressin V1b receptor antibody (ab104365)参考文献

ab104365 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Permeabilization Yes - 30min in citrate buffer @ 90oC
Specification Brain
Blocking step Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Emma Schofield

Verified customer

提交于 Sep 15 2016

Thank you for your inquiry.

I am happy to confirm that the immunogen of ab104365 shows less than 15% homology with VR1alpha receptor (3 residues out of 22).

According to this data it is extremely unlikely ab104365 is binding to VR1a...

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