概述

  • 产品名称Anti-Vasopressin抗体
    参阅全部 Vasopressin 一抗
  • 描述
    兔多克隆抗体to Vasopressin
  • 经测试应用适用于: IHC-FoFr, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Pig
  • 免疫原

    Full length native protein (purified)

性能

应用

Our Abpromise guarantee covers the use of ab39363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/5000.

靶标

  • 相关性Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • ADH antibody
    • Antidiuretic hormone antibody
    • Arginine vasopressin neurophysin II antibody
    • ARVP antibody
    • AVP antibody
    • AVP NPII antibody
    • copeptin antibody
    • Vasopressin neurophysin II copeptin antibody
    • VP antibody
    see all

Anti-Vasopressin antibody 图像

  • Minipigs were deeply anesthetized with a combination of midazolam and ketamine, prior to transcardial perfusion with phosphate buffered 4% paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
    Immunohistochemistry was performed using the avidin-biotin method. Accordingly, free-floating sections were first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with avidin (0.1%) and biotin (0.01%) were each of 10 minute duration and each procedure were followed by a 2 minute TBS-rinse. The sections were preincubated with 1% Triton X- 100 and 0.2% milk in TBS for 30 minutes, prior to incubation with the primary antibody, ab39363, for 72 hours at 4°C at a 1/100 dilution. The sections were then washed three times 15 minutes in TBS and 1% Triton X-100 prior to incubation for 1 hour at room temperature with the secondary anti-rabbit biotinylated antibody at a 1/400 with TBS that contained 1% Triton X- 100 and 0.2% milk. After a brief rinse in TBS, the endogenous peroxidase activity was blocked with a solution of 10 ml hydrogen peroxide, 10 ml methanol, and 80 ml TBS, for 10 minutes. The sections were then rinsed in TBS and 1% Triton for three times 15 minutes and incubated for 1 hour at room temperature with avidin-peroxidas diluted 1/400 in TBS that contained 1% Triton X-100 and 0.2% milk. The sections were then rinsed three times for 15 minutes in TBS and 1% Triton, prior to avidin-peroxidase visualization. The latter step consisted of incubation for 10 minutes in 10ml water in which one diaminobenzidine tablet had been dissolved and to which 10 ml of 35% H2O2 had been added. Finally, the sections were dehydrated, mounted and coverslipped with Depex.

    och: optic chiasm.

Anti-Vasopressin antibody (ab39363)参考文献

This product has been referenced in:
  • Mortensen AH  et al. Deletion of OTX2 in neural ectoderm delays anterior pituitary development. Hum Mol Genet 24:939-53 (2015). Read more (PubMed: 25315894) »
  • Zhao DQ & Ai HB Oxytocin and vasopressin involved in restraint water-immersion stress mediated by oxytocin receptor and vasopressin 1b receptor in rat brain. PLoS One 6:e23362 (2011). IHC-FrFl ; Rat . Read more (PubMed: 21858088) »

See all 6 Publications for this product

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Thank you for your enquiry.

Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

In this instance, none of our Vaso...

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Thank you for your inquiry. Unfortunately, we are unable to track down the particular antibodies the authors used when it is not specifically mentioned in the paper. However, the following antibodies might have been used: ab39363: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"