概述

  • 产品名称Anti-VASH1抗体
    参阅全部 VASH1 一抗
  • 描述
    小鼠单克隆抗体to VASH1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: GGKKVAGGGS SGATPTSAAA TAPSGVRRLE TSEGTSAQRD EEPEEEGEED LRDGGVPFFV NRGGLPVDEA TWERMWKHVA KIHPDGEKVA QRIRGATD, corresponding to amino acids 3-100 of Human VASH1 (NP_055724) with a proprietary tag.

  • 阳性对照
    • Tagged recombinant human VASH1 fragment (the immunogen). HepG2 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.
  • 组织特异性Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
  • 序列相似性Belongs to the vasohibin family.
  • 翻译后修饰2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1036 antibody
    • VASH antibody
    • VASH1 antibody
    • VASH1_HUMAN antibody
    • Vasohibin 1 antibody
    • Vasohibin-1 antibody
    see all

Anti-VASH1 antibody 图像

  • Anti-VASH1 antibody (ab88435) at 1 µg/ml + Tagged recombinant Human VASH1 fragment (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-VASH1 antibody (ab88435) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 41 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

Anti-VASH1 antibody (ab88435)参考文献

ab88435 has not yet been referenced specifically in any publications.

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