Anti-VAP1抗体(ab42885)

概述

  • 产品名称Anti-VAP1抗体
    参阅全部 VAP1 一抗
  • 描述
    兔多克隆抗体to VAP1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 150 - 250 of Rat VAP1.

    (Peptide available as ab42884.)

  • 阳性对照
    • This antibody gave a positive signal in the following Tissue Lysates: Lung (Rat), Lung (Mouse) and Lung (Human). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

性能

应用

Our Abpromise guarantee covers the use of ab42885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa).

靶标

  • 功能Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
  • 组织特异性Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
  • 序列相似性Belongs to the copper/topaquinone oxidase family.
  • 翻译后修饰Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
    N- and O-glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
    • AOC3 antibody
    • AOC3_HUMAN antibody
    • Copper amine oxidase antibody
    • HPAO antibody
    • Membrane copper amine oxidase antibody
    • Membrane primary amine oxidase antibody
    • Semicarbazide sensitive amine oxidase antibody
    • Semicarbazide-sensitive amine oxidase antibody
    • SSAO antibody
    • VAP-1 antibody
    • Vascular adhesion protein 1 antibody
    • VP97 antibody
    see all

Anti-VAP1 antibody 图像

  • IHC image of VAP1 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42885, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-VAP1 antibody (ab42885) at 1 µg/ml

    Lane 1 : Lung (Rat) Tissue Lysate
    Lane 2 : Lung (Mouse) Whole Cell Lysate - normal tissue
    Lane 3 : Lung (Human) Tissue Lysate - Fetal Normal Tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
    VAP1 has a predicted molecular weight of 85 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). The observed 100kDa band is consistent with other commercially avaliable antibodies.

Anti-VAP1 antibody (ab42885)参考文献

ab42885 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your reply.

ab81673 has been tested for use in WB and IP so the experiment you propose should be fine. An alternative is to use one VAP1 antibody for IP and second VAP1 antibody for WB.

I am sorry this product did not per...

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Thanks for your reply.

Perhaps it would be more efficient to discuss this issue on the phone. Please feel free to call me. Alternatively, if you provide a phone number I'm happy to give you a call.

I look forward to speaking with yo...

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Thanks for your email.

Since you have ab81673 I would recommend using it in a western blot with the 2 lysates you have been testing. It is quite likely this antibody will work in western blotting as it has already been shown to work in a dive...

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Thanks for your email.

The data you are seeing do appear unexpected.

Reducing conditions are used during western blotting.

Before I believe a loading control western blot was producing expected banding pattern so I don't t...

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Thanks for your reply.

While I'm happy to provide another vial of ab29297 or perhaps a different cell extract if you like, it appears that your in-house lung extract is working quite well. Perhaps it would be most efficient to continue using ...

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Thanks for your email and for including the western blot images.

There does appear to be differences between the lung lysate we sent you and your internal sample. From the GAPDH control the two lysates appear to be of good quality so I'm not ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1116832.

To check the status of the order please con...

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Thanks for your email.

I am happy to send another VAP1 antibody. Please let me know which anti-VAP1 antibody you would like as a replacement, as well as the order reference number or PO number used to purchase the initial vial of ab42885. Read More

Thank you for that information.

You should receive the vial of lysate to use as a positive control tomorrow. If after you receive the lysate, you are still not seeing the correct size band, then please let me know and I will be happy to look ...

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Thank you for calling Abcam earlier today.

I have been trying to see if there is a reason that you are getting a 75kDa band, instead of a band at 85kDa or 100kDa. I will be honest and say that I am unsure as to why that is occurring, but I wa...

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