概述

  • 产品名称Anti-VAMP8抗体[EP2629Y]
    参阅全部 VAMP8 一抗
  • 描述
    兔单克隆抗体[EP2629Y] to VAMP8
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VAMP8 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: 293T, HEK293, HeLa, RAW264.7, NIH/3T3 and PC-12 cell lysates and mouse kidney tissue lysate. IHC-P: Human brain and kidney tissue. ICC/IF: PC-12 cells. Flow Cyt: HeLa cells. IP: HEK293 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:

    Anti-VAMP8 antibody (HRP) [EP2629Y] (ab202547)

    Anti-VAMP8 antibody (Alexa Fluor® 488) [EP2629Y] (ab202828)

    Anti-VAMP8 antibody (Alexa Fluor® 647) [EP2629Y] (ab202829)

    Anti-VAMP8 antibody (Alexa Fluor® 405) [EP2629Y] (ab206354)

    Anti-VAMP8 antibody (Alexa Fluor® 594) [EP2629Y] (ab206587)

    Anti-VAMP8 antibody (Alexa Fluor® 555) [EP2629Y] (ab206588)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab76021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).
IP 1/20 - 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/80 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

靶标

  • 功能Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
  • 组织特异性Platelets.
  • 序列相似性Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EDB antibody
    • Endobrevin antibody
    • VAMP 8 antibody
    • VAMP-8 antibody
    • VAMP8 antibody
    • VAMP8_HUMAN antibody
    • Vesicle associated membrane protein 8 antibody
    • Vesicle-associated membrane protein 8 antibody
    see all

Anti-VAMP8 antibody [EP2629Y] 图像

  • All lanes : Anti-VAMP8 antibody [EP2629Y] (ab76021) at 1/10000 dilution (purified)

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 11 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Blocking and dilution buffer: 5% NFDM/TBST.
  • All lanes : Anti-VAMP8 antibody [EP2629Y] (ab76021) at 1/10000 dilution (purified)

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : RAW264.7 whole cell lysate
    Lane 4 : NIH/3T3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 11 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Blocking and dilution buffer: 5% NFDM/TBST.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling VAMP8 with purified ab76021 at a dilution of 1/250. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling VAMP8 with purified ab76021 at a dilution of 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Flow Cytometry analysis of HeLa cells labelling VAMP8 with purified ab76021 at a dilution of 1/150 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • ab76021 (purified) at 1/50 immunoprecipitating VAMP8 in HEK293 whole cell lysate.

    Lane 1 (input): HEK293 whole cell lysate (10µg)

    Lane 2 (+): ab76021 + HEK293 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76021 in HEK293 whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-VAMP8 antibody [EP2629Y] (ab76021) at 1/20000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 11 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling VAMP8 with unpurified ab76021 at a dilution of 1/100. A HRP/AP polymerized secondary antibody was used.

  • Immunohistochemical analysis of Human lacrimal gland tissue staining VAMP8 with unpurified ab76021.

    Antigen retrieval was performed using antigen retrieval solution in a microwave. Sections were blocked with 10 goat serum for 30 minutes and incubated with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB.

Anti-VAMP8 antibody [EP2629Y] (ab76021)参考文献

This product has been referenced in:
  • Xie X  et al. Deep vein thrombosis is accurately predicted by comprehensive analysis of the levels of microRNA-96 and plasma D-dimer. Exp Ther Med 12:1896-1900 (2016). WB ; Human . Read more (PubMed: 27588107) »
  • Chihara Y  et al. Diagnostic markers of urothelial cancer based on DNA methylation analysis. BMC Cancer 13:275 (2013). IHC-P ; Human . Read more (PubMed: 23735005) »

See all 3 Publications for this product

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