1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.
Golgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.
ab108051, at a 1/50 dilution, staining VAMP4 in formalin fixed, paraffin embedded Human hepatocarcinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.