重组Anti-VAMP2抗体[EPR12790] (ab181869)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12790] to VAMP2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-VAMP2抗体[EPR12790]
参阅全部 VAMP2 一抗 -
描述
兔单克隆抗体[EPR12790] to VAMP2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Human fetal brain and Human cerebellum lysates ICC/IF: SH-SY5Y cells. Flow Cyt (intra): Jurkat cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12790 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181869于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
For unpurified use at 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
|
ICC/IF |
1/250.
For unpurified use at 1/500. |
|
IP |
1/150.
For unpurified use at 1/70. |
说明 |
---|
Flow Cyt (Intra)
1/100. For unpurified use at 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa). |
ICC/IF
1/250. For unpurified use at 1/500. |
IP
1/150. For unpurified use at 1/70. |
靶标
-
功能
Involved in the targeting and/or fusion of transport vesicles to their target membrane. -
组织特异性
Nervous system and skeletal muscle. -
序列相似性
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
细胞定位
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles. - Information by UniProt
-
数据库链接
- Entrez Gene: 6844 Human
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- Omim: 185881 Human
- SwissProt: P63027 Human
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
- Unigene: 25348 Human
see all -
别名
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
图片
-
Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/10000 dilution (purified) + Rat heart lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 13 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
ab181869 staining VAMP2 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1:PBS only.
-
Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed) labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.
-
Intracellular Flow Cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with unpurified ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
-
Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution (purified) + Mouse brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 13 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution (purified) + Human cerebellum lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution (unpurified)
Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 13 kDa -
ab181869 (purified) at 1/150 immunoprecipitating VAMP2 in Human cerebellum whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
Intracellular Flow Cytometry analysis of SH-SY5Y cells labelling VAMP2 with purified ab181869 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
-
Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) cells labelling VAMP2 with purified ab181869 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
-
Immunofluorescent analysis of SH-SYSY cells (human cell line) (4% paraformaldehyde-fixed) labeling VAMP2 with unpurified ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with DAPI.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (13)
ab181869 被引用在 13 文献中.
- Ofori JK et al. Human Islet MicroRNA-200c Is Elevated in Type 2 Diabetes and Targets the Transcription Factor ETV5 to Reduce Insulin Secretion. Diabetes 71:275-284 (2022). PubMed: 34753799
- Kim JH et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun 12:6537 (2021). PubMed: 34764278
- Caló L et al. CSPa reduces aggregates and rescues striatal dopamine release in a-synuclein transgenic mice. Brain 144:1661-1669 (2021). PubMed: 33760024
- Kim K et al. Reduced Interaction of Aggregated a-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson's Disease. Genes (Basel) 12:N/A (2021). PubMed: 33801790
- Lamotte JD et al. hiPSC-Derived Neurons Provide a Robust and Physiologically Relevant In Vitro Platform to Test Botulinum Neurotoxins. Front Pharmacol 11:617867 (2020). PubMed: 33519485