概述

  • 产品名称
  • 描述
    兔多克隆抗体to VAMP2
  • 经测试应用
    适用于: WB, IP, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
    Sequence:

    MSATAATVPPAAPAGEGG


    (Peptide available as ab4958)

性能

应用

Our Abpromise guarantee covers the use of ab3347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).Can be blocked with VAMP2 peptide (ab4958).
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23056393

靶标

  • 功能
    Involved in the targeting and/or fusion of transport vesicles to their target membrane.
  • 组织特异性
    Nervous system and skeletal muscle.
  • 序列相似性
    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • 细胞定位
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

图片



  • Predicted band size : 19 kDa

    Western blot detection of VAMP2 on rat brain extract using ab3347.

  • VAMP2 antibody (ab3347) used in Immunohistochemistry (rat frozen sections). Sections were fixed with paraformaldehyde. Primary Antibody ab3347 used at 1/1000 incubated for 18 hours at 20°C in PBS-T 0.3%.  Secondary Antibody used anti-IgG Rabbit Conjugation Alexa Fluor® 488 Dilution 1/1000.

    See Abreview

  • Immunohistochemical analysis of rat spinal cord dorsal horn tissue, staining VAMP2 with ab3347 at 1/200 dilution. Fluorescent-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.

文献

This product has been referenced in:
  • Gordon SL  et al. A Fine Balance of Synaptophysin Levels Underlies Efficient Retrieval of Synaptobrevin II to Synaptic Vesicles. PLoS One 11:e0149457 (2016). Mouse . Read more (PubMed: 26871701) »
  • Takahashi N  et al. Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and ß cells. Nat Commun 6:8531 (2015). ICC/IF ; Mouse . Read more (PubMed: 26439845) »

See all 21 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification
hippocampus tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Jul 07 2014

Thank you for your reply and the additional information.

Please let me know your order or PO number. If the antibody was purchased within the last 6 months, our Abpromise guarantee applies and I'd be happy to offer you either a free of charge ...

Read More

Thank you for contacting us.
I am sorry to hear that the antibody is not working as expected.

I have copied the WB protocol below.
Also,
I have a few more questions in order to understand the issue better:
1) What is the exact...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Karine Thibault

Verified customer

提交于 Apr 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (hippocampal neuron)
Specification
hippocampal neuron
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2%
Username

Dr. Chanchoo Yap

Verified customer

提交于 Nov 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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