产品名称 Anti-VAMP1抗体 参阅全部 VAMP1 一抗
IHC-FoFr, IHC-P, WB more details
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat VAMP1.
(Peptide available as
WB: This antibody gave a positive signal in the following tissue lysates:
Mouse Brain, Mouse Spinal Cord, Rat Brain and Rat Spinal Cord.
IHC: rat brain
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明 IHC-FoFr: 1/3000.
WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能 Involved in the targeting and/or fusion of transport vesicles to their target membrane.
组织特异性 Nervous system, skeletal muscle and adipose tissue.
序列相似性 Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.
细胞定位 Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
Information by UniProt
SYB 1 antibody
Anti-VAMP1 antibody 图像
Western blot - VAMP1 antibody (ab41324)
All lanes : Anti-VAMP1 antibody (ab41324) at 1 µg/ml Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Brain (Rat) Tissue Lysate - normal tissue Lane 3 : Spinal Cord (Mouse) Tissue Lysate Lane 4 : Spinal Cord (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 13 kDa Observed band size : 17 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-VAMP1 antibody (ab41324) This image is courtesy of an abreview submitted by Sophie Pezet, CNRS, Paris, France
Immunohistochemistical detection of VAMP1 with ab41324 on rat brain PFA perfusion-fixed frozen sections. Primary antibody ab41324 diluted at 1/3000 and incubated for 18 hours at 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000)Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody stains synapses in many brain areas. The picture shows the staining taken at low power magnification, showing the staining in the hippocampus (top) and medium thalamus (bottom of the picture).
Anti-VAMP1 antibody (ab41324)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"