兔多克隆抗体to valyl tRNA synthetase
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab71291 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/100. Detects a band of approximately 135 kDa (predicted molecular weight: 141 kDa).
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain.
Information by UniProt
- Bat 6 antibody
- Bat6 antibody
- D17H6S56E antibody
Western blot - valyl tRNA synthetase antibody (ab71291)
Anti-valyl tRNA synthetase antibody (ab71291) at 1/60 dilution + HL60 cell line lysate at 35 µgPredicted band size :
141 kDaObserved band size :
135 kDa (why is the actual band size different from the predicted?
)Additional bands at :
55 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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