Anti-Vaccinia Virus抗体(ab35219)


  • 产品名称Anti-Vaccinia Virus抗体
    参阅全部 Vaccinia Virus 一抗
  • 描述
    兔多克隆抗体to Vaccinia Virus
  • 特异性This antibody reacts with purified Virions. It does not react with uninfected cells.
  • 经测试应用适用于: ELISA, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    Does not react with parainfluenza (1-3), RSV, Adeno, Influenza A and B or HSV (1). Reactive with the Lister, Wyeth, New York City and MVA strains of Vaccinia.
  • 免疫原

    Native virus, Lister strain.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯化说明This antibody is greater than 95% pure.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab35219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/500 - 1/2000.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734
IHC-P Use at an assay dependent concentration. PubMed: 25093734
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22615950


  • 相关性Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • 别名
    • A27L antibody
    • Orthopoxvirus antibody

Anti-Vaccinia Virus antibody (ab35219)参考文献

This product has been referenced in:
  • Larsen IV  et al. Both CD8+ and CD4+ T Cells Contribute to Corneal Clouding and Viral Clearance following Vaccinia Virus Infection in C57BL/6 Mice. J Virol 90:6557-72 (2016). IHC-P . Read more (PubMed: 27170749) »
  • Kober C  et al. Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps. J Transl Med 13:216 (2015). IHC . Read more (PubMed: 26149494) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cross section of mouse eye, looked at cornea)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA pH 9
Permeabilization No
Specification Cross section of mouse eye, looked at cornea
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Paraformaldehyde

Ms. Inna Larsen

Verified customer

提交于 Dec 29 2015

Unfortunately, none of the anti-Vaccinia Virus antibodies we have has been tested for cross-reactivity with Western Reserve Strain.

ab117453 has been tested and cross-reacts with 3 other Vaccinia strains and also with distantly relat...

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Thank you for contacting us.

We only have two monoclonal antibodies against the vaccinia virus in our catalogue.

I would recommend using ab21098 - Mouse monoclonal [TV46] to Vaccinia Virus ( to recognise A...

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