Anti-V5 tag抗体[SV5-Pk1] (ab27671)

概述

  • 产品名称
    Anti-V5 tag抗体[SV5-Pk1]
    参阅全部 V5 tag 一抗
  • 描述
    小鼠单克隆抗体[SV5-Pk1] to V5 tag
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details
  • 免疫原

    Mice were infected with the paramyxovirus SV5, Simian-Virus 5.

  • 表位
    ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
  • 常规说明

    ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody was purified from tissue culture supernatant by affinity chromatography.
  • Primary antibody说明
    Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
  • 克隆
    单克隆
  • 克隆编号
    SV5-Pk1
  • 骨髓瘤
    Sp2/0-Ag14
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21887337
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/1000 - 1/5000.
IP Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
RIA Use at an assay dependent concentration.

靶标

  • 相关性
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 别名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody [SV5-Pk1] 图像

  • Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution + mouse cortex cell line lysate at 30 µg

    Secondary
    Non-Abcam antibody was used: Vector Conjugation: HRP at 1/3000 dilution

    This image is courtesy of an abreview submitted by Melina Reisenberg, CARD, KCL, London

    See Abreview

  • ab27671 at a 1/1000 dilution staining V5 tag in CHO cells overexpressing a V5-tagged protein by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked with 3% BSA. The secondary used was an Alexa Fluor® 488 conjugated goat anti-mouse IgG (H+L) at a 1/1000 dilution.

Anti-V5 tag antibody [SV5-Pk1] (ab27671)参考文献

This product has been referenced in:
  • Moleirinho S  et al. Regulation of localization and function of the transcriptional co-activator YAP by angiomotin. Elife 6:N/A (2017). WB, IP ; Human . Read more (PubMed: 28464980) »
  • Ji H  et al. EGFR phosphorylates FAM129B to promote Ras activation. Proc Natl Acad Sci U S A 113:644-9 (2016). Read more (PubMed: 26721396) »

See all 30 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3 cells)
Gel Running Conditions
Reduced Denaturing (4%-12%)
Loading amount
20 µg
Treatment
Transfected with or without V5-tagged plasmids
Specification
NIH3T3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (myoblast)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
40 µg
Treatment
infected with retrovirus
Specification
myoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Miss. Can Zhou

Verified customer

提交于 Feb 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
vascular smooth muscle cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jan 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Human Cell (HEK293)
Specification
HEK293
Permeabilization
Yes - 0.2% triton
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 19 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your kinds words. I am happy to help!

As requested, I have issued a free of charge replacement for one vial of ab27671.

To check the status of the order please contact our Customer Service team and reference this numbe...

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Thanks for your reply. I am sorry to hear the replacement vial of ab3366 did not improve things.

Just to make sure, you would like me to send along one vial of ab27671 as a replacement for this most recent faulty vial of ab3366? If this is yo...

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For ab27671 we only have data on it being used in HEK cells and for ab9116, it was used in HeLa and the western blot data is available on the public website, in the Abreview section. Both antibodies appeared to give a strong signal in cells transfected...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cortex cell line)
Loading amount
30 µg
Specification
Cortex cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Nov 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Chinese Hamster Cultured Cells (CHO cells overexpressing a V5-tagged protein)
Specification
CHO cells overexpressing a V5-tagged protein
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 29 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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