概述

  • 产品名称
    Anti-V5 tag抗体(HRP)
    参阅全部 V5 tag 一抗
  • 描述
    兔多克隆抗体to V5 tag (HRP)
  • 偶联物
    HRP
  • 特异性
    In a direct ELISA against 1 ug of V5 peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • 经测试应用
    适用于: ICC, WB, ELISAmore details
  • 免疫原

    Synthetic peptide: GKPIPNPLLGLDST conjugated to KLH.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support and conjugated to horseradish peroxidase.
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 别名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

图片

  • All lanes : Anti-V5 tag antibody (HRP) (ab1325) at 0.2 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg


    Predicted band size : 1 kDa

文献

This product has been referenced in:
  • Gin P  et al. Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1. Hum Mol Genet 21:2961-72 (2012). ELISA . Read more (PubMed: 22493000) »
  • Levy D  et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). Read more (PubMed: 21131967) »

See all 2 Publications for this product

客户评价及客户问答

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Application
Western blot
Loading amount
0.1 µg
Gel Running Conditions
Reduced Denaturing (4~20%)
Sample
Escherichia coli Recombinant protein (Recombinant protein with C-terminus V5 tag)
Specification
Recombinant protein with C-terminus V5 tag
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (Recombinant V5 tagged Protein)
Specification
Recombinant V5 tagged Protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type
Direct
Username

Abcam user community

Verified customer

提交于 Aug 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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