概述

  • 产品名称Anti-V5 tag抗体(HRP)
    参阅全部 V5 tag 一抗
  • 描述
    兔多克隆抗体to V5 tag (HRP)
  • 偶联物HRP
  • 特异性In a direct ELISA against 1 ug of V5 peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • 经测试应用适用于: ICC, WB, ELISAmore details
  • 免疫原

    Synthetic peptide:

    GKPIPNPLLGLDST

    conjugated to KLH.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support and conjugated to horseradish peroxidase.
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent dilution.

靶标

  • 相关性The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 别名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody (HRP) (ab1325)参考文献

This product has been referenced in:
  • Gin P  et al. Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1. Hum Mol Genet 21:2961-72 (2012). ELISA . Read more (PubMed: 22493000) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 0.1 µg
Gel Running Conditions Reduced Denaturing (4~20%)
Sample Escherichia coli Recombinant protein (Recombinant protein with C-terminus V5 tag)
Specification Recombinant protein with C-terminus V5 tag
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 30 2014

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab15828. To check the status of the order please contact our Customer...

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Thank you for your enquiry. I am sorry to hear this vial of ab1325 is giving you trouble. It does appear that this antibody is not detecting the V5 tag as expected. If you have purchased it within the last 6 months or so I would be happy to offer you a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (Recombinant V5 tagged Protein)
Specification Recombinant V5 tagged Protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type Direct
Username

Abcam user community

Verified customer

提交于 Aug 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"