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Our Abpromise guarantee covers the use of ab53418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/500 - 1/2000.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000 - 1/3000. Detects a band of approximately 130 kDa.|
|ELISA||1/5000 - 1/10000.|
|IHC-Fr||1/500 - 1/2000.|
ab53418 staining V5 tag in Mouse B16F1 melanoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Tween 20 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 1% BSA + 0.5% Tween 20 + 1% Donkey serum) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Melonma cells expressing GFP are also expressing GFP (AF55) due to transfected cell line with GFP-V5.