Anti-V5 tag抗体- ChIP Grade (ab9116)

概述

  • 产品名称
    Anti-V5 tag抗体- ChIP Grade
    参阅全部 V5 tag 一抗
  • 描述
    兔多克隆抗体to V5 tag - ChIP Grade
  • 特异性
    ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
  • 经测试应用
    适用于: ICC/IF, ChIP, WB, ELISA, IP, ChIP/Chip, IHC-P, ICCmore details
  • 免疫原

    GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.

  • 阳性对照
    • Ab5395

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg .
  • 克隆
    多克隆
  • 同种型
    IgG
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 - 10 µg/ml. PubMed: 18575576
ChIP Use at an assay dependent concentration. PubMed: 17289569
WB Use a concentration of 0.05 - 0.5 µg/ml. Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ChIP/Chip Use at an assay dependent concentration. PubMed: 17289569
IHC-P Use a concentration of 0.5 - 5 µg/ml.
ICC Use at an assay dependent concentration. PubMed: 17012257

靶标

  • 相关性
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 别名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

图片

  • All lanes : Anti-V5 tag antibody - ChIP Grade (ab9116) at 1/5000 dilution

    Lane 1 : Cells transfected with v5-tagged protein.
    Lane 2 : Cells transfected with v5-tagged protein.
    Lane 3 : Cells transfected with v5-tagged protein.
    Lane 4 : Untransfected cells.
    Lane 5 : Untransfected cells.

    Secondary
    Lanes 1 - 3 : Anti-rabbit Ig-HRP at 1/20000 dilution
    Lanes 4 - 5 : Anti-rabbit Ig-HRP at 1/20000 dilution

    This image is courtesy of an anonymous Abreview

    Mouse ES cells lysate.

    See Abreview

  • ab9116 staining V5 tag in the Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 3% serum for 1 hour at 4°C. Samples were incubated with primary antibody (1/300) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/300).

    See Abreview

  • ChIP qPCR: The graph on the left represents the input chromatin of PC3-EMT14-OVOL2 relative to empty vector (EV) control. The graph on the right depicts the ChIP DNA using V5 antibody (ab9116). The V5 epitope was included at the C-terminus of the expressed OVOL2. Results were normalized to input controls and graphs are relative to EV. Graphs show mean +/- sem.

  • ab9116 at 1/1000 dilution staining V5 in V5-Pgc transfected Drosophila Kc cells by Immunocytochemistry/ Immunofluorescence. Kc cells were fixed in 4% formaldehyde for 10 minutes, washed in PBS-Tween 20 (0.05%) and blocked in 5% milk in PBS-Tween 20 for 1 hour. Cells were incubated with primary antibody at 4°C overnight and the secondary antibody at room temperature for 3 hours.

文献

This product has been referenced in:

See all 59 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
U2OS cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (H1299)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Treatment
transfected with V-5 p53
Specification
H1299
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 28 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human u2os cells)
Permeabilization
Yes - 0.1% triton
Specification
human u2os cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 13 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH/3T3 fibroblasts with V5-tag constructs)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NIH/3T3 fibroblasts with V5-tag constructs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Pawel Niewiadomski

Verified customer

提交于 Feb 09 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
HEK293
Blocking step
Thermo Scientific #EN37543 Starting Block TBST formulation as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Monkey Cell (cos7 cell)
Specification
cos7 cell
Permeabilization
Yes - 0.2% tritonx
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 19 2013


Nous n’avons pas testé cet anticorps dans nos laboratoires et donc nous n’avons pas de dilution spécifique à recommander pour ab9116 appart les informations dans la publication suivante :
Sugiyama T et al. SHREC, an effector complex for heter...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Recombinant protein (293 cells transfected with a V5-tagged construct)
Loading amount
2000 cells
Specification
293 cells transfected with a V5-tagged construct
Gel Running Conditions
Reduced Denaturing (10% AA gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 19 2012

Thank you for calling today, and I'm sorry to hear that our V5 antibody is giving you so much trouble!

As we discussed over the phone, I'm unsure of why the antibody is detecting that band in your samples and all of the protocol modifications ...

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I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. The code will give you the VALUE of each product off an order before the expiration date. To redeem this offer, please submit an Abreview for flow c...

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