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GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
Our Abpromise guarantee covers the use of ab9116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 10 µg/ml. PubMed: 18575576|
|ChIP||Use at an assay dependent concentration. PubMed: 17289569|
|WB||Use a concentration of 0.05 - 0.5 µg/ml. Can be blocked with V5 tag peptide (ab15829).|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ChIP/Chip||Use at an assay dependent concentration. PubMed: 17289569|
|IHC-P||Use a concentration of 0.5 - 5 µg/ml.|
|ICC||Use at an assay dependent concentration. PubMed: 17012257|
ab9116 staining V5 tag in the Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 3% serum for 1 hour at 4°C. Samples were incubated with primary antibody (1/300) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/300).
ChIP qPCR: The graph on the left represents the input chromatin of PC3-EMT14-OVOL2 relative to empty vector (EV) control. The graph on the right depicts the ChIP DNA using V5 antibody (ab9116). The V5 epitope was included at the C-terminus of the expressed OVOL2. Results were normalized to input controls and graphs are relative to EV. Graphs show mean +/- sem.
ab9116 at 1/1000 dilution staining V5 in V5-Pgc transfected Drosophila Kc cells by Immunocytochemistry/ Immunofluorescence. Kc cells were fixed in 4% formaldehyde for 10 minutes, washed in PBS-Tween 20 (0.05%) and blocked in 5% milk in PBS-Tween 20 for 1 hour. Cells were incubated with primary antibody at 4°C overnight and the secondary antibody at room temperature for 3 hours.