Use at a dilution of: Coating: 1:100 - 1:500 Primary: 1:1000 - 1:30,000.
Use at an assay dependent concentration.
1/200 - 1/2000.
IHC - Wholemount
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
GKPIPNPLLGLDST epitope tag antibody
GKPIPNPLLGLDST tag antibody
Protein Rev antibody
Regulator of expression of viral proteins antibody
V5 epitope tag antibody
Western blot - Anti-V5 tag antibody (ab9137)
All lanes : Anti-V5 tag antibody (ab9137) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate at 0.2 µg Lane 2 : E. coli whole cell lysate at 0.1 µg Lane 3 : E. coli whole cell lysate at 0.05 µg