概述

  • 产品名称Anti-V5 tag抗体
    参阅全部 V5 tag 一抗
  • 描述
    鸡多克隆抗体to V5 tag
  • 特异性Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:100,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP conjugated goat anti chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
  • 经测试应用适用于: ELISA, WB, ICC/IF, ICCmore details
  • 免疫原

    GKPIPNPLLGLDST

    (V5 epitope) conjugated with KLH.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 纯度IgY fraction
  • 纯化说明Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. Antibody concentration was determined by extinction coefficient : O.D. 1.32 at 280nM equals 1.0 mg of IgY (IgG).
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab9113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB 1/5000. Can be blocked with V5 tag peptide (ab15829).
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

靶标

  • 相关性The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 别名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody 图像

  • ab9113 staining human fibrosarcoma cells (HT1080) by ICC/IF. Cells were transfected with V5- tagged transmembrane protein and PFA fixed without undergoing permeabilization and blocking steps. The primary antibody was used at 10µg/ml and incubated with the sample for 1 hour at 20°C. An Alexa Fluor® 546 conjugated donkey polyclonal anti-chicken (H+L) antibody, diluted 1/500, was used as the secondary.

    See Abreview

Anti-V5 tag antibody (ab9113)参考文献

This product has been referenced in:
  • Chen X  et al. Coronavirus membrane-associated papain-like proteases induce autophagy through interacting with Beclin1 to negatively regulate antiviral innate immunity. Protein Cell 5:912-27 (2014). IF ; Human . Read more (PubMed: 25311841) »
  • Stanton RJ  et al. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. J Clin Invest 120:3191-208 (2010). WB . Read more (PubMed: 20679731) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

I can confirm that ab24740 Myc tag antibody and ab9113 V5 tag antibody will be suitable for WB and ICC-IF experiments.

As you will be doing duel staining, you will need to use primary antibodies from di...

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Thank you for your response. Some researchers add the cryoprotectant glycerol to a final concentration of 50% to prevent freeze/thaw damage; glycerol will lower the freezing point to below -20oC. While this may be acceptable for many antibodies...

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Thank you for your enquiry regarding ab9113. Every product has specific storage instruction and we would recommend storing this particular antibody at 4ᵒC. Since the storage buffer does not contain any cryoprotective agent, lower temperature than 4...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization No
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提交于 Dec 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"