概述

  • 产品名称
  • 描述
    鸡多克隆抗体to V5 tag
  • 经测试应用
    适用于: WBmore details
  • 免疫原

    Synthetic peptide:

    GKPIPNPLLGLDST(C)

    (V5 epitope) conjugated to KLH. (Antibody raised against peptide available as ab15829.)

  • 阳性对照
    • This antibody gave a positive signal in Drosophila S2 cells transfected with V5 epitope tagged protein. No band was observed in the negative control.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 3% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab107244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Block using 3% milk.

靶标

  • 相关性
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 别名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

图片

  • All lanes : Anti-V5 tag antibody (ab107244) at 1 µg/ml

    Lane 1 : Drosophila S2 cells transfected with V5 epitope tagged MSI-A
    Lane 2 : Drosophila S2 Negative Control

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

实验方案

文献

ab107244 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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