重组
RabMAb

Anti-v-Myb + c-Myb (phospho S11)抗体[EP769Y] - ChIP Grade (ab45150)

概述

  • 产品名称
    Anti-v-Myb + c-Myb (phospho S11)抗体[EP769Y] - ChIP Grade
    参阅全部 v-Myb + c-Myb 一抗
  • 描述
    兔单克隆抗体[EP769Y] to v-Myb + c-Myb (phospho S11) - ChIP Grade
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IP, ChIPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminus of human c-Myb.

  • 阳性对照
    • IHC-P: human cervical carcinoma tissue.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab45150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
IP 1/50.
ChIP Use at an assay dependent concentration. PubMed: 21296997
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 相关性
      Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
    • 细胞定位
      Nuclear
    • 数据库链接
    • 别名
      • C myb antibody
      • C myb protein antibody
      • Cmyb antibody
      • MYB antibody
      • Myb proto oncogene antibody
      • V myb myeloblastosis viral oncogene homolog antibody
      see all

    图片

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/2000 dilution (purified)

      Lane 1 : Molt-4 cell lysate with v-Myb + c-Myb (phospho S11) peptide
      Lane 2 : Molt-4 cell lysate with v-Myb + c-Myb (phospho S12) peptide
      Lane 3 : Molt-4 cell lysate with v-Myb + c-Myb (phospho S11/12) peptide
      Lane 4 : Molt-4 cell lysate with v-Myb + c-Myb unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/2000 dilution (purified)

      Lane 1 : HeLa cell lysate with v-Myb + c-Myc (phospho S11) peptide
      Lane 2 : HeLa cell lysate with v-Myb + c-Myc (phospho S12) peptide
      Lane 3 : HeLa cell lysate with v-Myb + c-Myc (phospho S11/12) peptide
      Lane 4 : HeLa cell lysate with v-Myb + c-Myc unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size : 72 kDa
      Observed band size : 75,77 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/5000 dilution (purified)

      Lane 1 : Untreated Ramos cell lysate
      Lane 2 : Ramos cell lysate, membrane treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 75,77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Dot blot analysis of v-Myb + c-Myb (phospho S11/12) peptide (Lane 1) and v-Myb + c-Myb unmodified peptide (Lane 2) labelling v-Myb + c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/5000 dilution (purified)

      Lane 1 : Untreated HL-60 cell lysate
      Lane 2 : HL-60 cell lysate, membrane treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/2000 dilution (purified)

      Lane 1 : untreated HeLa cell lysate
      Lane 2 : HeLa cell lysate, the membrane treated with lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size : 72 kDa
      Observed band size : 75,77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150) at 1/10000 dilution (unpurified) + Ramos membrane

      Predicted band size : 72 kDa
      Observed band size : 72,75 kDa (why is the actual band size different from the predicted?)
    • Unpurified ab45150 staining human v-Myb / c-Myb in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.

    文献

    This product has been referenced in:
    • Abraham BJ  et al. Small genomic insertions form enhancers that misregulate oncogenes. Nat Commun 8:14385 (2017). CHIPseq ; Human . Read more (PubMed: 28181482) »
    • Prinzhorn W  et al. c-MYB is a transcriptional regulator of ESPL1/Separase in BCR-ABL-positive chronic myeloid leukemia. Biomark Res 4:5 (2016). WB . Read more (PubMed: 26937281) »

    See all 19 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (chronic myelogenous leukemia & monkey kidney cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris (BioRad))
    Loading amount
    50000 cells
    Specification
    chronic myelogenous leukemia & monkey kidney cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Ragnhild Eskeland

    Verified customer

    提交于 Apr 22 2015

    Thank you for contacting us.

    This product is raised against the N-terminal region. It should not be affected by a truncated C-terminus. We do guarantee this to recognizeAvian myeloblastosis viral (v Myb) in human in the following applicati...

    Read More

    Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

    Die Protokolldetails bestätigen meine Vermutung, dass der anti-Myb-Antikörper wohl leider fehlerhaft ist. Zwar ist die Lyse mit Ultraschall vielleicht etwa...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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