概述

  • 产品名称Anti-UXT抗体
    参阅全部 UXT 一抗
  • 描述
    山羊多克隆抗体to UXT
  • 特异性ab77483 is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1).
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-DHRDKVYEQLAKY

    , from an internal region of Human UXT (NP_004173.1; NP_705582.1).

  • 阳性对照
    • Human Kidney and Skeletal Muscle lysates.

性能

应用

Our Abpromise guarantee covers the use of ab77483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor transcription (AR). AR N-terminus-associated coactivator which may play a role in facilitating receptor-induced transcriptional activation (PubMed:11854421). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed:11827465). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed:17554592). May be a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function (PubMed:17620405). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed:17635584). Together with URI1, associates with chromatin to the NKX3-1 promoter region.
  • 组织特异性Ubiquitous. Expressed in prostate epithelial cells. Overexpressed in a number of tumor tissues (PubMed:16221885). Highest levels in the heart, skeletal muscle, pancreas, kidney, liver, adrenal gland, peripheral blood leukocytes, lymph node, prostate, and thyroid and the lowest levels in bladder and uterus.
  • 序列相似性Belongs to the UXT family.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen receptor trapped clone 27 protein antibody
    • ART 27 antibody
    • ART-27 antibody
    • OTTHUMP00000023226 antibody
    • OTTHUMP00000023227 antibody
    • Protein UXT antibody
    • SKP2 associated alpha PFD 1 antibody
    • STAP1 antibody
    • Ubiquitously expressed prefoldin like chaperone antibody
    • Ubiquitously expressed transcript antibody
    • Ubiquitously expressed transcript protein antibody
    • Uxt antibody
    • UXT_HUMAN antibody
    see all

Anti-UXT antibody 图像

  • ab77483 (5µg/ml) staining of UXT in paraffin embedded Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of cystoplasm in all epithelial cells, but nuclear staining in some of them.

  • Anti-UXT antibody (ab77483) at 0.03 µg/ml + Human Kidney lysate (in RIPA buffer) at 35 µg

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-UXT antibody (ab77483)参考文献

ab77483 has not yet been referenced specifically in any publications.

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