Anti-UTY抗体(ab85969)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to UTY
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 775 and 825 of human UTY (NP_009056.3)

  • 阳性对照
    • Jurkat whole cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab85969 was affinity purified using an epitope specific to UTY immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Histone demethylase.
    • 序列相似性
      Belongs to the UTX family.
      Contains 1 JmjC domain.
      Contains 8 TPR repeats.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Histone demethylase UTY antibody
      • Ubiquitously transcribed TPR protein on the Y chromosome antibody
      • Ubiquitously transcribed Y chromosome tetratricopeptide repeat protein antibody
      • Ubiquitously-transcribed TPR protein on the Y chromosome antibody
      • Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein antibody
      • UTY antibody
      • UTY_HUMAN antibody
      see all

    图片

    • All lanes : Anti-UTY antibody (ab85969) at 0.04 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : Jurkat whole cell lysate at 5 µg


      Predicted band size : 150 kDa
      Observed band size : 165 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of UTY by Western Blot of Immunprecipitate.
      ab85969 at 1µg/ml staining UTY in Jurkat whole cell lysate immunoprecipitated using ab85969 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    文献

    ab85969 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you very much for your interest in ab85969.

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