概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab36655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml.

靶标

  • 功能
    Histone demethylase.
  • 序列相似性
    Belongs to the UTX family.
    Contains 1 JmjC domain.
    Contains 8 TPR repeats.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Histone demethylase UTY antibody
    • Ubiquitously transcribed TPR protein on the Y chromosome antibody
    • Ubiquitously transcribed Y chromosome tetratricopeptide repeat protein antibody
    • Ubiquitously-transcribed TPR protein on the Y chromosome antibody
    • Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein antibody
    • UTY antibody
    • UTY_HUMAN antibody
    see all

Anti-UTY antibody 图像

  • IHC image of UTY staining in human lymphoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36655, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-UTY antibody (ab36655)参考文献

ab36655 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human and mouse cell lysate (see figure))
Loading amount
20 µg
Specification
Human and mouse cell lysate (see figure)
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
5% milk in PBS + 0.1% Tween as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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