UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.
ICC/IF image of ab24273 stained mouse embryonic stem cells. The cells were PFA fixed (4% PFA, 20 min) and incubated with the antibody (ab24273, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
The large nuclei of the feeder cells can be seen in the image; ab24273 does not localise to these nuclei. ab24273 can be seen localising to the much smaller nuclei of the mouse embryonic stem cells.
Western blot - Anti-UTF1 antibody (ab24273)
All lanes : Anti-UTF1 antibody (ab24273) at 1 µg/ml
Lane 1 : Mouse Embryonic Stem Cell Lysate Lane 2 : Mouse Embryonic Stem Cell Lysate with Human UTF1 peptide (ab30369) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution
ab24273 detected a band of the predicted size of UTF1 in Mouse Embryonic Stem Cell lysate. The band was blocked using the immunising peptide, ab30369. An additional band of unknown identity was also recognized by the antibody.