概述

  • 产品名称Anti-UTF1抗体
    参阅全部 UTF1 一抗
  • 描述
    兔多克隆抗体to UTF1
  • 经测试应用适用于: IHC-P, WB, ICC/IF, ChIPmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Mouse UTF1.

  • 阳性对照
    • Mouse embryonic stem cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab24273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 22266011
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 1 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 24672748

靶标

  • 功能Acts as a transcriptional coactivator of ATF2.
  • 结构域The leucine-zipper domain is required for coactivation activity. When this domain is deleted, the protein is able to stimulate transcription from a number of gene promoters.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.
  • 别名
    • hUTF 1 antibody
    • hUTF1 antibody
    • Undifferentiated embryonic cell transcription factor 1 antibody
    • UTF 1 antibody
    • UTF1 antibody
    • UTF1_HUMAN antibody
    see all

Anti-UTF1 antibody 图像

  • ICC/IF image of ab24273 stained mouse embryonic stem cells. The cells were PFA fixed (4% PFA, 20 min) and incubated with the antibody (ab24273, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).

    The large nuclei of the feeder cells can be seen in the image; ab24273 does not localise to these nuclei. ab24273 can be seen localising to the much smaller nuclei of the mouse embryonic stem cells.

  • All lanes : Anti-UTF1 antibody (ab24273) at 1 µg/ml

    Lane 1 : Mouse Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate with Human UTF1 peptide (ab30369) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 46 kDa (possible non-specific binding,post-translational modification).

Anti-UTF1 antibody (ab24273)参考文献

This product has been referenced in:
  • Galonska C  et al. In Vivo and in vitro dynamics of undifferentiated embryonic cell transcription factor 1. Stem Cell Reports 2:245-52 (2014). ChIP ; Mouse . Read more (PubMed: 24672748) »
  • Vidal SE  et al. Combinatorial modulation of signaling pathways reveals cell-type-specific requirements for highly efficient and synchronous iPSC reprogramming. Stem Cell Reports 3:574-84 (2014). ICC/IF ; Mouse . Read more (PubMed: 25358786) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic)
Permeabilization Yes - Triton X-100 (0.5%)
Specification Embryonic
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Oct 23 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Mr. Utkarsh Kapoor

Verified customer

提交于 Dec 28 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP on chip
Sample Mouse Cell lysate - other (male germ cell culture)
Specification male germ cell culture
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Microarray
Positive control The sample we used for the ChIP pulldown with this antibody was cultured mouse male germ cells with confirmed UTF1 expression by ICC. 30ug chromatin and 5ug antibody used per ChIP reaction.
Negative control Input - gDNA from chromatin from same sample.
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Abcam user community

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提交于 Jul 30 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate + 0.05% Tween-20
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jul 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse ES Cell (wt D3 line) undifferentiated)
Specification Mouse ES Cell (wt D3 line) undifferentiated
Fixative Paraformaldehyde
Permeabilization Yes - see blocking buffer
Blocking step 1% serum, 0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Dec 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells)
Loading amount 40 µg
Specification Mouse Embryonic Stem Cells
Gel Running Conditions Reduced Denaturing (12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

提交于 Aug 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"