Anti-UTF1抗体(ab105090)

概述

  • 产品名称Anti-UTF1抗体
    参阅全部 UTF1 一抗
  • 描述
    兔多克隆抗体to UTF1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Human, Pig, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human UTF1.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: E14Tg2a; F9; IOUD2.

性能

应用

Our Abpromise guarantee covers the use of ab105090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

靶标

  • 功能Acts as a transcriptional coactivator of ATF2.
  • 结构域The leucine-zipper domain is required for coactivation activity. When this domain is deleted, the protein is able to stimulate transcription from a number of gene promoters.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.
  • 别名
    • hUTF 1 antibody
    • hUTF1 antibody
    • Undifferentiated embryonic cell transcription factor 1 antibody
    • UTF 1 antibody
    • UTF1 antibody
    • UTF1_HUMAN antibody
    see all

Anti-UTF1 antibody 图像

  • All lanes : Anti-UTF1 antibody (ab105090) at 1 µg/ml

    Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab105090 stained mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) mouse ES cells at 1µg/ml.

Anti-UTF1 antibody (ab105090)参考文献

ab105090 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Human Cell lysate - whole cell (undifferentiated human iPS cells)
Specification undifferentiated human iPS cells
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Jan 22 2014

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Expiration date: January 11th. 2013

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Undifferentiated mouse ES cells)
Specification Undifferentiated mouse ES cells
Fixative Paraformaldehyde
Permeabilization Yes - see blocking buffer
Blocking step 1% serum, 0.1% triton, 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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提交于 Jan 16 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Undifferentiated human ES cells grown on feeders)
Specification Undifferentiated human ES cells grown on feeders
Fixative Paraformaldehyde
Permeabilization Yes - see blocking buffer
Blocking step 1% serum, 0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1%
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提交于 Dec 23 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP on chip
Sample Mouse Cell lysate - other (male germ cell culture)
Specification male germ cell culture
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Microarray
Positive control Sample for positive pulldown with this antibody was cultured mouse male germ cells with confirmed UTF1 expression by ICC. 30ug chromatin and 5ug antibody used per ChIP reaction.
Negative control Input - gDNA from chromatin from same sample.
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提交于 Dec 15 2011

Thank you for your phone call. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab105090 in ChIP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To rede...

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