概述

  • 产品名称
    Anti-Uteroglobin抗体
    参阅全部 Uteroglobin 一抗
  • 描述
    兔多克隆抗体to Uteroglobin
  • 经测试应用
    适用于: IHC-P, ELISA, ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant rat uteroglobin His fusion protein.

  • 常规说明


    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1600.
ELISA Use at an assay dependent concentration. PubMed: 21787397
ICC/IF Use at an assay dependent concentration. PubMed: 24367567
WB 1/5000. Predicted molecular weight: 10 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • 组织特异性
    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • 序列相似性
    Belongs to the secretoglobin family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Blastokinin antibody
    • CC10 antibody
    • CC16 antibody
    • CCPBP antibody
    • CCSP antibody
    • Clara cell phospholipid binding protein antibody
    • Clara cell phospholipid-binding protein antibody
    • Clara cell specific 10 kD protein antibody
    • Clara cells 10 kDa secretory protein antibody
    • OTTHUMP00000236107 antibody
    • SCGB1A1 antibody
    • Secretoglobin family 1A member 1 antibody
    • Secretoglobin, family 1A, member 1 (uteroglobin) antibody
    • UG antibody
    • UGB antibody
    • UP-1 antibody
    • UP1 antibody
    • Urinary protein 1 antibody
    • Urine protein 1 antibody
    • UTER_HUMAN antibody
    • Uteroglobin antibody
    see all

Anti-Uteroglobin antibody 图像

  • Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).

  • ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image. 

  • All lanes : Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution

    Lane 1 : Mouse lung whole tissue lysate with Milk
    Lane 2 : Mouse kidney whole tissue lysate with Milk

    Lysates/proteins at 20 µg per lane.
    Blocking peptides at 5 % per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

Anti-Uteroglobin antibody (ab40873)参考文献

This product has been referenced in:
  • Strupp C  et al. Relationship of Metabolism and Cell Proliferation to the Mode of Action of Fluensulfone-Induced Mouse Lung Tumors. II: Additional Mechanistic Studies. Toxicol Sci 154:296-308 (2016). Read more (PubMed: 27605420) »
  • Crabbé A  et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One 10:e0126846 (2015). IHC-P ; Mouse . Read more (PubMed: 25962111) »

See all 14 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

提交于 Feb 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung, bronchial epithelium)
Permeabilization
No
Specification
lung, bronchial epithelium
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (urine)
Loading amount
5 µg
Specification
urine
Treatment
CLB (NP-40 based lysis buffer with protease inhibitors) , 10 min on ice
Gel Running Conditions
Reduced Denaturing (18)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Lung Parenchyma)
Specification
Human Lung Parenchyma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
1% BSA with 0.1% Triton-X in PBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Abcam user community

Verified customer

提交于 Mar 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin (Ab970) 0.37%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Sep 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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