概述

  • 产品名称Anti-Uteroglobin抗体
    参阅全部 Uteroglobin 一抗
  • 描述
    兔多克隆抗体to Uteroglobin
  • 经测试应用适用于: IHC-P, ELISA, ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant rat uteroglobin His fusion protein.

  • 常规说明


    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1600.
ELISA Use at an assay dependent concentration. PubMed: 21787397
ICC/IF Use at an assay dependent concentration. PubMed: 24367567
WB 1/5000. Predicted molecular weight: 10 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • 组织特异性Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • 序列相似性Belongs to the secretoglobin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Blastokinin antibody
    • CC10 antibody
    • CC16 antibody
    • CCPBP antibody
    • CCSP antibody
    • Clara cell phospholipid binding protein antibody
    • Clara cell phospholipid-binding protein antibody
    • Clara cell specific 10 kD protein antibody
    • Clara cells 10 kDa secretory protein antibody
    • OTTHUMP00000236107 antibody
    • SCGB1A1 antibody
    • Secretoglobin family 1A member 1 antibody
    • Secretoglobin, family 1A, member 1 (uteroglobin) antibody
    • UG antibody
    • UGB antibody
    • UP-1 antibody
    • UP1 antibody
    • Urinary protein 1 antibody
    • Urine protein 1 antibody
    • UTER_HUMAN antibody
    • Uteroglobin antibody
    see all

Anti-Uteroglobin antibody 图像

  • Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).

  • ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image. 

  • Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution + Wild-type adult mouse lung homogenate was used.

    Secondary
    5% Dry milk, 1 hour at room temperature at 1/5000 dilution

    Predicted band size : 10 kDa
    Observed band size : 10 kDa
  • ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

Anti-Uteroglobin antibody (ab40873)参考文献

This product has been referenced in:
  • Crabbé A  et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One 10:e0126846 (2015). IHC-P ; Mouse . Read more (PubMed: 25962111) »
  • Woik N  et al. Kelch-like ECT2-interacting protein KLEIP regulates late-stage pulmonary maturation via Hif-2a in mice. Dis Model Mech 7:683-92 (2014). Read more (PubMed: 24785085) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (urine)
Loading amount 5 µg
Specification urine
Treatment CLB (NP-40 based lysis buffer with protease inhibitors) , 10 min on ice
Gel Running Conditions Reduced Denaturing (18)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

提交于 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Lung Parenchyma)
Specification Human Lung Parenchyma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step 1% BSA with 0.1% Triton-X in PBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 18°C
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Abcam user community

Verified customer

提交于 Mar 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin (Ab970) 0.37%
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Sep 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"