概述

  • 产品名称Anti-USP9x抗体
    参阅全部 USP9x 一抗
  • 描述
    兔多克隆抗体to USP9x
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Cow, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 625-675 of Human USP9x (CAD18900.2).

  • 阳性对照
    • HeLa, 293T and NIH 3T3 whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab99343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 289 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.
  • 组织特异性Widely expressed in embryonic and adult tissues.
  • 序列相似性Belongs to the peptidase C19 family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme FAF X antibody
    • Deubiquitinating enzyme FAF-X antibody
    • DFFRX antibody
    • Drosophila fat facets related X linked antibody
    • FAF antibody
    • Fafl antibody
    • Fam antibody
    • Fat facets homolog antibody
    • Fat facets in mammals antibody
    • Fat facets protein related X linked antibody
    • Fat facets protein related, X-linked antibody
    • Fat facets protein-related antibody
    • hFAM antibody
    • MRX99 antibody
    • Probable ubiquitin carboxyl terminal hydrolase FAF X antibody
    • Probable ubiquitin carboxyl-terminal hydrolase FAF-X antibody
    • Ubiquitin carboxyl-terminal hydrolase FAM antibody
    • Ubiquitin specific peptidase 9 X linked antibody
    • Ubiquitin specific peptidase 9, X-linked antibody
    • Ubiquitin specific processing protease FAF X antibody
    • Ubiquitin specific protease 9 X chromosome antibody
    • Ubiquitin thioesterase FAF X antibody
    • Ubiquitin thiolesterase FAF X antibody
    • Ubiquitin thiolesterase FAF-X antibody
    • Ubiquitin-specific protease 9 antibody
    • Ubiquitin-specific-processing protease FAF-X antibody
    • USP9 (gene name) antibody
    • Usp9x antibody
    • USP9X_HUMAN antibody
    • Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila) antibody
    • X chromosome antibody
    • X-linked antibody
    see all

Anti-USP9x antibody 图像

  • All lanes : Anti-USP9x antibody (ab99343) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 289 kDa


    Exposure time : 30 seconds
  • Detection of USP9x in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99343 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-USP9x antibody (ab99343)参考文献

This product has been referenced in:
  • Paemka L  et al. Seizures are regulated by ubiquitin-specific peptidase 9 X-linked (USP9X), a de-ubiquitinase. PLoS Genet 11:e1005022 (2015). Read more (PubMed: 25763846) »
  • Oosterkamp HM  et al. USP9X downregulation renders breast cancer cells resistant to tamoxifen. Cancer Res 74:3810-20 (2014). WB ; Human . Read more (PubMed: 25028367) »

See all 2 Publications for this product

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