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Synthetic peptide conjugated to KLH derived from within residues 2500 to the C-terminus of Human USP9x.
(Peptide available as ab20618.)
Our Abpromise guarantee covers the use of ab26334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 54, 105, 289 kDa (predicted molecular weight: 292 kDa).|
|IHC-P||1/450. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab26334 stained HeLa cells. The cells were 100% Methanol fixed (5min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26334, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Image courtesy of Human Protein Atlas
ab26334 staining USP9x in Human stomach. The paraffin embedded human skin tissue was incubated with ab26334 (1/450 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab26334 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab26334 has not yet been referenced specifically in any publications.