Anti-USP9x抗体(ab26334)

概述

  • 产品名称Anti-USP9x抗体
    参阅全部 USP9x 一抗
  • 描述
    兔多克隆抗体to USP9x
  • 特异性USP9x exists as the full-length protein as well as several fragmented proteins: 289kDa Human protein: Q93008 USP9X protein (Full length) 105kDa Human protein: Q6P468 - USP9X protein (Fragment) 99.7kDa Human protein: Q59EZ5 - USP9X protein variant (Fragment). 53.9kDa Human protein: Q86X58 - USP9X protein (Fragment). ab26334 detects the full-length protein at ~290kDa as well as the 105 and 54kDa fragments. All detected bands are quenched by the immunizing peptide ab20618. Immunizing peptide has 87% homology with USP9Y, which may be recognized by ab26334
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: IHC (PFA fixed) or IHC-Fr
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 2500 to the C-terminus of Human USP9x.

    (Peptide available as ab20618.)

  • 阳性对照
    • USP9x can be detected in the polarized intestinal cell line T84 which is a human colon carcinoma epithelial cell line. In tissue culture: USP9x is also observed in L-cell fibroblasts and MDCK cells. USP9x is ubiquitously expressed in all adult tissues. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab26334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54, 105, 289 kDa (predicted molecular weight: 292 kDa).
IHC-P 1/450. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
  • 应用说明Is unsuitable for IHC (PFA fixed) or IHC-Fr.
  • 靶标

    • 功能Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.
    • 组织特异性Widely expressed in embryonic and adult tissues.
    • 序列相似性Belongs to the peptidase C19 family.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Deubiquitinating enzyme FAF X antibody
      • Deubiquitinating enzyme FAF-X antibody
      • DFFRX antibody
      • Drosophila fat facets related X linked antibody
      • FAF antibody
      • Fafl antibody
      • Fam antibody
      • Fat facets homolog antibody
      • Fat facets in mammals antibody
      • Fat facets protein related X linked antibody
      • Fat facets protein related, X-linked antibody
      • Fat facets protein-related antibody
      • hFAM antibody
      • MRX99 antibody
      • Probable ubiquitin carboxyl terminal hydrolase FAF X antibody
      • Probable ubiquitin carboxyl-terminal hydrolase FAF-X antibody
      • Ubiquitin carboxyl-terminal hydrolase FAM antibody
      • Ubiquitin specific peptidase 9 X linked antibody
      • Ubiquitin specific peptidase 9, X-linked antibody
      • Ubiquitin specific processing protease FAF X antibody
      • Ubiquitin specific protease 9 X chromosome antibody
      • Ubiquitin thioesterase FAF X antibody
      • Ubiquitin thiolesterase FAF X antibody
      • Ubiquitin thiolesterase FAF-X antibody
      • Ubiquitin-specific protease 9 antibody
      • Ubiquitin-specific-processing protease FAF-X antibody
      • USP9 (gene name) antibody
      • Usp9x antibody
      • USP9X_HUMAN antibody
      • Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila) antibody
      • X chromosome antibody
      • X-linked antibody
      see all

    Anti-USP9x antibody 图像

    • ICC/IF image of ab26334 stained HeLa cells. The cells were 100% Methanol fixed (5min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26334, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Image courtesy of Human Protein Atlas

      ab26334 staining USP9x in Human stomach. The paraffin embedded human skin tissue was incubated with ab26334 (1/450 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab26334 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

      Further results for this antibody can be found at www.proteinatlas.org

    Anti-USP9x antibody (ab26334)参考文献

    ab26334 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Rat brain sections)
    Specification Rat brain sections
    Fixative Paraformaldehyde
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 May 18 2006

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