概述

  • 产品名称Anti-USP6抗体
    参阅全部 USP6 一抗
  • 描述
    山羊多克隆抗体to USP6
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    KISPLHHLQMECSP

    , corresponding to C terminal amino acids 773-786 of Human USP6.

  • 阳性对照
    • Human placenta
  • 常规说明GenBank Accession Number – NP_004496. Gene Ontology terms - lysosomal cysteine-type endopeptidase; nucleic acid binding; deubiquitination; oncogenesis; protein modification.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab4553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).Can be blocked with Human USP6 peptide (ab23054). An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

靶标

  • 功能Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
  • 组织特异性Testis specific. Expressed in various cancer cell lines.
  • 疾病相关Note=A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 1 Rab-GAP TBC domain.
  • 结构域The Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6.
  • 翻译后修饰Monubiquitinated; ubiquitination is calmodulin and calcium dependent.
  • 细胞定位Cell membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 6 antibody
    • HRP1 antibody
    • Proto-oncogene TRE-2 antibody
    • TRE17 antibody
    • TRE2 antibody
    • Ubiquitin carboxyl-terminal hydrolase 6 antibody
    • Ubiquitin specific protease 6 antibody
    • Ubiquitin thiolesterase 6 antibody
    • Ubiquitin-specific-processing protease 6 antibody
    • UBP6_HUMAN antibody
    • USP6 antibody
    see all

Anti-USP6 antibody 图像

  • All lanes : Anti-USP6 antibody (ab4553) at 2 µg/ml

    Lane 1 : Human placenta lysate in RIPA buffer
    Lane 2 : Human placenta lysate in RIPA buffer with immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
    Additional bands at : 50 kDa (possible non-specific binding).

Anti-USP6 antibody (ab4553)参考文献

This product has been referenced in:
  • Urso ML  et al. Analysis of human skeletal muscle after 48 h immobilization reveals alterations in mRNA and protein for extracellular matrix components. J Appl Physiol 101:1136-48 (2006). Read more (PubMed: 16763108) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. This antibody is guaranted for use in WB for Human samples. The antibody was used at a concentration of 1-3 ug/ml, incubated overnight at 4 degrees. Blocking was done with 5% BSA. There is a non-specific band that appears a...

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Thank you for your enquiry. ab4553 is a fast track antibody. I have enclosed the fast track policy below. Essentially it is an antibody that we already sell at a reduced price since it is not fully characterized, therefore, I cannot send you a samp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"