Horse, Pig, Gorilla
Recombinant fragment: LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEP, corresponding to internal sequence amino acids 211-343 of Human USP51 (UniProt ID: Q70EK9).
- IHC-P: Human pancreas tissue
WB: Human RT-4, U-251 MG, A-431, liver, and tonsil lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Expressed in prostate, brain, lung, aorta and kidney.
Belongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger.
Information by UniProt
- Deubiquitinating enzyme 51 antibody
- Ubiquitin carboxyl-terminal hydrolase 51 antibody
- Ubiquitin specific peptidase 51 antibody
Immunocytochemistry/ Immunofluorescence - Anti-USP51 antibody (ab121147)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP51 antibody (ab121147)
Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab121147 at a 1/100 dilution. The image shows strong cytoplasmic positivity in exocrine glandular cells.
has not yet been referenced specifically in any publications.
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