Anti-USP51抗体(ab121147)

概述

  • 产品名称
    Anti-USP51抗体
  • 描述
    兔多克隆抗体to USP51
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Pig, Gorilla
  • 免疫原

    Recombinant fragment: LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEP, corresponding to internal sequence amino acids 211-343 of Human USP51 (UniProt ID: Q70EK9).

  • 阳性对照
    • IHC-P: Human pancreas tissue WB: Human RT-4, U-251 MG, A-431, liver, and tonsil lysates

性能

应用

Our Abpromise guarantee covers the use of ab121147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 组织特异性
    Expressed in prostate, brain, lung, aorta and kidney.
  • 序列相似性
    Belongs to the peptidase C19 family.
    Contains 1 UBP-type zinc finger.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 51 antibody
    • Ubiquitin carboxyl-terminal hydrolase 51 antibody
    • Ubiquitin specific peptidase 51 antibody
    • Ubiquitin thioesterase 51 antibody
    • Ubiquitin thiolesterase 51 antibody
    • Ubiquitin-specific-processing protease 51 antibody
    • UBP51_HUMAN antibody
    • USP51 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab121147 at a 1/100 dilution. The image shows strong cytoplasmic positivity in exocrine glandular cells.

文献

ab121147 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Feb 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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